BIO-701 / 2 crédits

Enseignant(s): Abriata Luciano Andres, Hacker David, Lau Kelvin Ka Ching, Pojer Florence

Langue: Anglais

Remark: Every year


Every 2 years


Cultivated animal cells are important hosts for the production of recombinant proteins for biochemical and structural studies and for use as therapeutics. The course will provide an overview of the methods for the production and characterization of recombinant proteins.


Subjects discussed in class are expected to include:

  • Transient gene expression
  • Stable gene expression
  • Cell-free protein synthesis
  • Production of therapeutic antibodies
  • Production of transmembrane proteins
  • X-ray structure determination
  • Cryogenic electron microscopy
  • Bio-NMR
  • Biophysical techniques for protein characterization

Other topics may be included as needed. Lectures will be presented by the instructors and other experts at the EPFL and in the Lémanic region. Each doctoral student will present a 45-minute talk on a specific subject within the field.


This course will take place in autumn 2025, exact dates and place to be confirmed.

Max. 20 participants. To register, please send an email to


recombinant protein, mammalian cells, cell culture, gene expression, transfection. protein structure

Learning Prerequisites

Required courses

Basic cell biology

Learning Outcomes

By the end of the course, the student must be able to:

  • Explain (i.e. comprehend) technologies discussed in course
  • Interpret in a critical way data generated with these technologies

Assessment methods

Oral presentation


Moodle Link

Dans les plans d'études

  • Nombre de places: 15
  • Forme de l'examen: Exposé (session libre)
  • Matière examinée: Recombinant protein expression in animal cells for applications in medicine and structural biology
  • Cours: 28 Heure(s)
  • Type: optionnel

Semaine de référence

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