Lab on cell-free synthetic biology
EE-490(j) / 4 crédits
Enseignant: Maerkl Sebastian
Langue: Anglais
Withdrawal: It is not allowed to withdraw from this subject after the registration deadline.
Summary
The cell-free synthetic biology course introduces engineers to the most commonly used techniques required to conduct work in Biotechnology and Bioengineering. Additionally, this course is an experiment in democratizing education and open science by generating useful resources for the local community
Content
Note: registration for this course is limited to a maximum of 16 students
The hands-on Cell-free synthetic biology laboratory course will introduce engineering students to fundamental methods and techniques commonly used in Biotechnology and Bioengineering. In this course the students will generate a cell-free transcription and translation system called the PURE system, which entails learning and performing polymerase chain reactions (PCRs), protein overexpression, protein purification, and other important molecular biology techniques. The students will then assess the functionality of their PURE systems by quantifying the expression of a green fluorescent protein (GFP).
Cell-free systems are becoming exceedingly powerful and therefore popular tools in biotechnology, bioengineering, and synthetic biology. In one recent example the 2019 EPFL iGEM team who won the iGEM competition that year, developed a cell-free molecular diagnostic kit for the identification and differentiation of two grapevine diseases (see first link below). In addition, we have shown in the past that cell-free systems are ideal tools for education and teaching by providing local high-schools with cell-free educational kits (see second link below). The cell-free synthetic biology course is therefore exploring the possibility to leverage the ample resources available in higher education to generate an additional, synergistic benefit for the population. We are hoping that with this course we will be able to sustainably produce cell-free educational kits for use in local schools.
The cell-free synthetic biology course will consist of a couple of introductory lectures, followed by laboratory experiments. The students will be guided through all the required steps required for each specific method and technique to generate functional cell-free systems (see third link below for a video guided tutorial published by our laboratory describing the steps required to generate a cell-free system). At the end of course, if we succeeded to produce functional cell-free systems we plan to package them into kits together with the students of the class and distribute them to interested local high-schools for their use in their biology curriculum.
Keywords
molecular biology
laboratory techniques and methods
biotechnology
bioengineering
synthetic biology
cell-free synthetic biology
democratizing education
open science
Learning Prerequisites
Required courses
There are no pre-requisite courses.
No prior experience or background in biology is required.
Recommended courses
EE-526 Introduction to Bioengineering
Learning Outcomes
By the end of the course, the student must be able to:
- Analyze experimental outcomes
- Carry out out molecular biology techniques
- Conduct molecular biology experiments
- Quantify the functionality of a cell-free system
- Create cell-free systems
Teaching methods
The course will be primarily hands-on experimental work, with real-time instructions and feedback.
Assessment methods
Students will be assessed by their ability to conduct molecular biology experiements, maintain a laboratory notebook, critically assess and report the outcomes of experiments, and work in a team.
Supervision
Office hours | Yes |
Assistants | Yes |
Forum | Yes |
Dans les plans d'études
- Semestre: Automne
- Nombre de places: 16
- Forme de l'examen: Pendant le semestre (session d'hiver)
- Matière examinée: Lab on cell-free synthetic biology
- TP: 4 Heure(s) hebdo x 14 semaines
- Type: optionnel
- Semestre: Automne
- Nombre de places: 16
- Forme de l'examen: Pendant le semestre (session d'hiver)
- Matière examinée: Lab on cell-free synthetic biology
- TP: 4 Heure(s) hebdo x 14 semaines
- Type: optionnel
- Semestre: Automne
- Nombre de places: 16
- Forme de l'examen: Pendant le semestre (session d'hiver)
- Matière examinée: Lab on cell-free synthetic biology
- TP: 4 Heure(s) hebdo x 14 semaines
- Type: optionnel
- Semestre: Automne
- Nombre de places: 16
- Forme de l'examen: Pendant le semestre (session d'hiver)
- Matière examinée: Lab on cell-free synthetic biology
- TP: 4 Heure(s) hebdo x 14 semaines
- Type: optionnel
Semaine de référence
Lu | Ma | Me | Je | Ve | |
8-9 | |||||
9-10 | |||||
10-11 | |||||
11-12 | |||||
12-13 | |||||
13-14 | |||||
14-15 | |||||
15-16 | |||||
16-17 | |||||
17-18 | |||||
18-19 | |||||
19-20 | |||||
20-21 | |||||
21-22 |
Légendes:
Cours
Exercice, TP
Projet, labo, autre
Mardi, 14h - 18h: Exercice, TP