Practical - Antanasijevic Lab
BIO-614 / 1 credit
Teacher(s): Antanasijevic Aleksandar, Eray Elise Rose
Language: English
Remark: Open to max. 4 students. 3-day block course, every year in January. To register, contact EDMS Administration
Frequency
Every year
Summary
The students will learn: 1) how to handle viral protein antigens and antibody samples 2) how to assemble and purify immune complexes using liquid chromatography 3) how to image them on an electron microscope
Content
Structural analyses of antibody-antigen complexes are essential for understanding the molecular mechanisms underlying the recognition and antibody-mediated inhibition of viral pathogens.
Through this course, the students will learn the basics of viral antigen handling, purification of antibody-antigen complexes and their characterization using electron microscopy (EM).
The key practical skills that students will get introduced to are liquid chromatography, preparation of EM grids and collection of data on the microscope.
Note
Please note that you are not allowed to inscribe in your own group!
Keywords
Virus, antigen, antibody, electron microscopy, structural biology
Learning Prerequisites
Required courses
No prerequisites
Learning Outcomes
By the end of the course, the student must be able to:
- understand the fundamental concept of the performed experiments and to communicate their outcome.
Assessment methods
Project report
Resources
Websites
Moodle Link
In the programs
- Number of places: 4
- Exam form: Project report (session free)
- Subject examined: Practical - Antanasijevic Lab
- Lecture: 6 Hour(s)
- Exercises: 2 Hour(s)
- Project: 8 Hour(s)
- Practical work: 8 Hour(s)
- Type: optional